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KMID : 0604520120380030225
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Journal of the Society of Cosmetic Scientists of Korea
2012 Volume.38 No. 3 p.225 ~ p.236
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Cellular Protective and Antioxidative Acivities of Parthenocissus tricuspidata Stem Extracts
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Jo Na-Rae
Park Min-A
Chae Kyo-Young
Park Su-Ah
Jeon So-Ha
Ha Ji-Hoon
Park Soo-Nam
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Abstract
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In this study, the cellular protective effects on HaCaT cells and human erythrocytes and antioxidative effects of P. tricuspidata stem extracts were investigated. The ethyl acetate (50 μ g/mL) and aglycone fraction (25 μg/mL) of P. tricuspidata stem extracts doesn't show any characteristics of cytotoxicity. When HaCaT cells were treated with 10 mM H2O2 and 30 μ M rose bengal, the ethyl acetate (6.25 ∼ 50 μ g/mL) and aglycone (6.25 ∼ 25 μ g/mL) fraction protected the cells against the oxidative damage in a concentration dependent manner. The P. tricuspidata stem extracts showed more prominent cellular protective effect than (+)-α-tocopherol, known as lipid antioxidant at 10 μ g/mL. The ethylacetate fraction of P. tricuspidata stem extracts (18.5 μ g/mL) showed more free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC550). Reactive oxygen species (ROS) scavenging activity (OSC50) of P. tricuspidata stem extracts on ROS generated in Fe3+-EDTA/H2O2 system was investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate (1.72 μ g/mL) and the aglycone fraction (1.53 μ g/mL) showed similar ROS scavenging activity of L-ascorbic acid (1.50 μ g/mL). These results indicate that extract/fractions of P. tricuspidata stem extracts can function as natural cytoprotective agents and antioxidants in biological systems, particularly skin exposed to UV radiation by protecting cellular membrane against ROS.
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KEYWORD
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parthenocissus tricuspidata stem extracts, cellular protective effect, reactive oxygen species, antioxidative activity
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